TABLE 4.
Effect of resistance-conferring mutations on nucleoside analog potency in cell-free assays
| NS5B | 2′-C-Methyl-DAPN-TP |
2′-C-Methyl-GTP |
2′-C-Methyl-2′-F-UTP |
|||
|---|---|---|---|---|---|---|
| IC50a (μM) | FDb | IC50 (μM) | FD | IC50 (μM) | FD | |
| GT 1b WT | 3.4 ± 1.1 | 1 | 5.6 ± 1.6 | 1 | 2.9 ± 0.6 | 1 |
| GT 1b S96T | 2.5 ± 0.6 | 0.7 | 8.8 ± 2.9 | 1.6 | 3.5 ± 0.9 | 1.2 |
| GT 1b S282T | >50 | >15 | >50 | >9 | >50 | >19 |
a
In vitro IC50s were determined with cell-free assays using purified recombinant NS5BΔ21. Each value is the average from two to three replicates (± standard deviations).
b
FD, fold difference.