. 2016 Jul 22;60(8):4809–4819. doi: 10.1128/AAC.00713-16

TABLE 4.

Relative constitutive expression levels of genes associated with xenobiotic metabolism and transport in IVM-selected (IVR10) and MOX-selected C. elegans strains compared with the wild-type Bristol N2 strain^c

Category	Gene	Mean fold change in constitutive gene expression relative to wild-type Bristol N2 ± SD
Category	Gene	IVM-selected strain (IVR10)	MOX-selected strain
P-glycoproteins (ABCB subfamily)	Cel-pgp1	2.16 ± 0.15^a	2.96 ± 0.43^a^,^b
	Cel-pgp2	1.59 ± 0.07^a	1.25 ± 0.11^a^,^b
	Cel-pgp3	1.90 ± 0.28^a	1.90 ± 0.15^a
	Cel-pgp4	0.86 ± 0.05	0.78 ± 0.03
	Cel-pgp5	1.74 ± 0.20^a	1.66 ± 0.11^a
	Cel-pgp6	4.77 ± 0.69^a	3.20 ± 0.41^a^,^b
	Cel-pgp7	1.08 ± 0.09	1.00 ± 0.11
	Cel-pgp8	1.26 ± 0.14	1.51 ± 0.25^a
	Cel-pgp9	2.13 ± 0.13^a	2.06 ± 0.21^a
	Cel-pgp10	1.37 ± 0.13^a	1.24 ± 0.08
	Cel-pgp11	1.43 ± 0.13^a	1.40 ± 0.08^a
	Cel-pgp12	1.76 ± 0.31^a	1.78 ± 0.18^a
	Cel-pgp13	1.36 ± 0.21	1.22 ± 0.11
	Cel-pgp14	4.26 ± 0.35^a	2.06 ± 0.23^a^,^b
Multidrug resistance-associated proteins (MRPs) (ABCC subfamily)	Cel-mrp1	1.41 ± 0.13^a	1.12 ± 0.17
	Cel-mrp2	1.46 ± 0.29	1.23 ± 0.13
	Cel-mrp3	2.21 ± 0.40^a	1.77 ± 0.12^a
	Cel-mrp4	1.05 ± 0.14	1.00 ± 0.01
	Cel-mrp5	1.09 ± 0.13	1.06 ± 0.07
	Cel-mrp6	1.79 ± 0.14^a	1.48 ± 0.03^a
	Cel-mrp7	1.18 ± 0.20	1.22 ± 0.04
	Cel-mrp8	1.41 ± 0.12^a	1.17 ± 0.04^b
Mitochondrial half-molecular ABC transporters	Cel-haf1	0.99 ± 0.05	1.01 ± 0.08
	Cel-haf2	1.08 ± 0.17	0.97 ± 0.05
	Cel-haf3	1.22 ± 0.20	1.09 ± 0.02
	Cel-haf4	2.12 ± 0.29^a	1.60 ± 0.02^a^,^b
	Cel-haf6	1.35 ± 0.20	1.14 ± 0.11
	Cel-haf7	1.39 ± 0.26	1.36 ± 0.15
	Cel-haf8	1.08 ± 0.12	0.91 ± 0.13
	Cel-haf9	2.00 ± 0.12^a	1.70 ± 0.10^a
Peroxisomal membrane protein related (putative ABCD transporter subfamily)	Cel-pmp1	1.10 ± 0.09	1.26 ± 0.15
	Cel-pmp2	1.07 ± 0.04	1.14 ± 0.06
	Cel-pmp3	0.89 ± 0.02	0.98 ± 0.07
	Cel-pmp4	1.57 ± 0.08^a	1.34 ± 0.20^a
	Cel-pmp5	1.73 ± 0.13^a	1.73 ± 0.09^a
Detoxification enzymes (glutamate-cysteine ligase and glutathione S-transferases)	Cel-gcs1	1.05 ± 0.23	0.81 ± 0.09
	Cel-gst1	1.23 ± 0.21	0.92 ± 0.11
	Cel-gst2	1.52 ± 0.31	1.45 ± 0.16
	Cel-gst4	2.14 ± 0.27^a	1.70 ± 0.57
	Cel-gst5	2.03 ± 0.36^a	1.73 ± 0.52
	Cel-gst7	1.07 ± 0.19	0.94 ± 0.07
	Cel-gst10	1.54 ± 0.25	1.70 ± 0.47
Cytochromes P450	Cel-cyp13A1	2.60 ± 1.00	2.10 ± 0.61
	Cel-cyp14A1	1.66 ± 0.47	1.50 ± 0.19
	Cel-cyp14A2	3.26 ± 0.42^a	2.31 ± 0.39^a^,^b
	Cel-cyp14A5	3.06 ± 0.39^a	1.96 ± 0.38^a^,^b
	Cel-cyp25A1	0.74 ± 0.23	0.98 ± 0.38
	Cel-cyp25A2	1.19 ± 0.20	1.29 ± 0.07
	Cel-cyp25A3	1.46 ± 0.57	1.03 ± 0.06
	Cel-cyp35A1	1.77 ± 0.49	4.78 ± 0.26^a^,^b
	Cel-cyp35A2	1.33 ± 0.34	1.49 ± 0.42
	Cel-cyp35A5	0.68 ± 0.12	1.02 ± 0.19
	Cel-cyp35C1	0.78 ± 0.08	1.08 ± 0.16
	Cel-cyp37B1	5.69 ± 0.75^a	1.89 ± 0.46^b

Significantly different from the wild-type Bristol N2 strain (P < 0.05).

Significantly different from the IVM-selected strain (P < 0.05).

Data are expressed as fold changes relative to the wild-type Bristol N2 strain and are reported as the means ± SD of data from two to four independent experiments. Boldface type indicates upregulated genes.