FIG 4.

Effect of compound treatment on VLP trafficking. HeLa cells treated with 50 μM of the indicated compounds for 1 h were incubated with EBOV VLPs containing VP40-GFP for 2.5 h. Fixed cells were permeabilized and stained for EEA1 (an early endosome marker) and LAMP1 (a lysosome marker). 3D modeling of deconvolved image stacks was done using Imaris software. The portion of VLPs colocalized with EEA1 (A) or LAMP1 (B) is shown as the average ± SD for more than 200 cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001.