FIG 3.
Dose response of POL7001 against P. aeruginosa in a mouse model of acute airway infection in comparison with CIP. C57BL/6 male mice (8 to 10 weeks of age) were infected i.t. with 1 × 107 CFU of planktonic MDR-RP73. After MDR-RP73 infection, saline solution, 2 or 8 mg/kg CIP, or different doses of POL7001 (0.5, 2, and 10 mg/kg) were administered 15 min postinfection by the i.t. route. Mouse lungs were collected after 24 h, homogenized, and plated on TSA plates to determine bacterial load. Dots represent measurements from individual mice, and horizontal lines represent the mean values. The data are pooled from two or three independent experiments (n = 6 to 14). Statistical significance determined by one-way ANOVA followed by Dunnett's analysis is indicated as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001.