TABLE 3.
Cytokines and chemokines after P. aeruginosa acute and chronic airway infection and antibiotic treatmenta
| Infection category, treatment, and cytokine or chemokine | Level (mean pg/ml ± SEM) |
P value |
|||
|---|---|---|---|---|---|
| Saline solution | CIP (8 mg/kg) | POL7001 (2 mg/kg) | Saline solution vs CIP | Saline solution vs POL7001 | |
| MDR-RP73 acute infection—i.t. treatment | |||||
| IL-1β | 7,068 ± 1,337 | 4,300 ± 1,137 | 4,203 ± 1,422 | ns | ns |
| CXCL1/KC | 74,255 ± 11,474 | 19,480 ± 4,793 | 22,591 ± 3,790 | <0.001 | <0.001 |
| CXCL2/MIP-2 | 111,456 ± 14,596 | 34,093 ± 8,157 | 31,750 ± 6,019 | <0.001 | <0.001 |
| CCL2/JE | 8,731 ± 1,609 | 3,275 ± 397.7 | 4,085 ± 1,170 | <0.05 | <0.05 |
| MDR-RP73 chronic infection—aerosol treatment | |||||
| IL-1β | 1,034 ± 7.575 | 838.2 ± 70.26 | 586.8 ± 158.5 | ns | <0.01 |
| CXCL1/KC | 33,174 ± 9,638 | 9,186 ± 2,849 | 5,145 ± 1,789 | <0.01 | <0.01 |
| CXCL2/MIP-2 | 14,919 ± 3,351 | 2,859 ± 573.8 | 2,279 ± 414.5 | <0.001 | <0.001 |
| CCL2/JE | 12,269 ± 1,916 | 11,269 ± 1,558 | 8,577 ± 1,160 | ns | ns |
a
C57BL/6 male mice (8 to 10 weeks old) were infected with MDR-RP73 and treated according to the schedule depicted in Fig. 1. IL-1β, CXCL1/KC, CXCL2/MIP-2, and CCL2/JE levels in lung homogenates were measured by enzyme-linked immunosorbent assay (ELISA) after 24 h or 7 days of P. aeruginosa acute and chronic infection. Data represent mean values ± standard errors of the means (SEM) of results from mice (n = 4 to 11) pooled from two to three independent experiments. Statistical significance as determined by one-way ANOVA followed by Dunnett's analysis is indicated. ns, not significant.