TABLE 5.

Cytokines and chemokines after P. aeruginosa RP73 chronic airway infection in CF and non-CF mice treated with POL7001 by pulmonary administration^a

Cytokine or chemokine	Level (under conditions of MDR-RP73 chronic infection—aerosol treatment) (pg/ml [mean ± SEM])				P value
	Non-CF—saline solution	CF—saline solution	Non-CF—POL7001 (2 mg/kg)	CF—POL7001 (2 mg/kg)	Non-CF saline solution vs CF saline solution	Non-CF POL vs CF POL	Non-CF saline solution vs non-CF POL	CF saline solution vs CF POL
IL-1β	1,684 ± 262.5	1,691 ± 305.3	1,280 ± 231.5	598.5 ± 257.3	ns	ns	ns	<0.05
CXCL1/KC	19,776 ± 2,460	13,210 ± 2,284	14,167 ± 1,833	11,989 ± 1,442	ns	ns	ns	ns
CXCL2/MIP-2	9,577 ± 2,604	8,419 ± 2,625	1,087 ± 165.4	1,282 ± 286.8	ns	ns	<0.01	<0.05
CCL2/JE	2,352 ± 467	2,574 ± 795.1	1,718 ± 394	1,232 ± 252.6	ns	ns	ns	ns

^aC57BL/6 Cftr^tm1UNCTgN(FABPCFTR)#Jaw (CF) mice and the corresponding wt congenic mice (non-CF) (11 to 18 weeks of age) were infected with MDR-RP73 embedded in agar beads and treated with saline solution or POL7001 (2 mg/kg) by the use of a Penn-Century device according to the schedule shown in Fig. 1B. Lungs were collected after 7 days of infection, and IL-1β, CXCL1/KC, CXCL2/MIP-2, and CCL2/JE levels were measured by ELISA using supernatants of lung homogenates. Data represent mean values ± SEM. The data are pooled from the results determined for mice from two independent experiments (n = 4 to 8). Statistical significance determined by Mann-Whitney U test analysis is indicated. ns, not significant.