Skip to main content
. 2016 Jul 22;60(8):4646–4651. doi: 10.1128/AAC.03025-15

TABLE 1.

Assay parameters

Parameter Description or value
Assay type Photometric
Reaction mode Rate up
Primary wavelength 340 nm
Secondary wavelength 412 nm
Last read required 26
Absorbance range 0.0000–0.0000
Main read time 18–26 s
Flex read time 0–0
Color correction read time 0–0
Blank read time <empty>
Sample blank type None
Blank assay <empty>
Reagent TOBRASI
R1 reagent vol 174 µl
R2 reagent vol 87 µl
R1 water vol 0 µl
R2 water vol 0 µl
R1 dispense mode Type 0
R2 dispense mode Type 0
Diluent name Water
Diluent dispense mode Type 0