TABLE 1.
EC50s of SbIII and AgNO3 and corresponding SIs for wild-type or LbAQP1-overexpressing Leishmania (Viannia) speciesa
| Leishmania line | SbIII |
AgNO3 |
||
|---|---|---|---|---|
| EC50 (95% CI) (μM) | SI | EC50 (95% CI) (μM) | SI | |
| L. guyanensis WT | 48.72 (43.09–55.08) | 1.37 (1.25–1.49) | ||
| L. guyanensis/pIR1-BSD | 48.96 (42.79–56.02) | 1.4 (1.28–1.51) | ||
| L. guyanensis/pIR1-BSD-AQP1 cl 16 | 30.09 (27.21–33.26)** | 0.61 | 1.62 (1.47–1.8)* | 1.18 |
| L. guyanensis/pIR1-BSD-AQP1 cl 18 | 29.50 (26.12–33.31)** | 0.6 | 1.61 (1.48–1.74)* | 1.17 |
| L. braziliensis WT | 108 (103.3–112.9) | 0.95 (0.88–1.03) | ||
| L. braziliensis/pIR1-BSD | 94.21 (88.06–100.8) | 0.94 (0.86–1.02) | ||
| L. braziliensis/pIR1-BSD-AQP1 cl 12 | 40.94. (37.94–44.17)*** | 0.38 | 1.5 (1.4–1.62)*** | 1.56 |
| L. braziliensis/pIR1-BSD-AQP1 cl 15 | 46 (43.05–49.13)*** | 0.42 | 1.57 (1.47–1.67)*** | 1.65 |
a
Abbreviations and symbols: CI, confidence interval; cl, clone; SI, susceptibility index, which is equal to the EC50 for the LgAQP1-overexpressing isolate/EC50 for the WT isolate; ***, P < 0.0001; **, P = 0.0012; *, P = 0.0121 (analysis of variance). EC50s are averages from at least two independent experiments performed in triplicate and were obtained after 48 h of exposure to potassium antimonyl tartrate, which was used as the SbIII source (Sigma-Aldrich, St. Louis, MO, USA), or AgNO3 (silver nitrate).