FIG 3.

Excitation (A, C) and emission (B, D) spectra illustrating the interactions between high-molecular-weight soluble β-glucan and EO-OPE-DABCO (A, B) or PPE-DABCO (bottom, D). Dashed lines represent the spectra of PE compounds alone, and solid lines represent the spectra observed in PE–β-glucan mixtures. The inset in panel D illustrates fluorescent PPE-DABCO concentrated in the cell wall of a C. albicans yeast cell, as shown in Fig. S2 in the supplemental material. Fl. Int. (ct/s), fluorescence intensity (counts/second). Additionally, confocal microscopy images illustrate PPE-DABCO bound to glucan microparticles. A transmitted light image (E) and reflected light image (F) are shown, with 405-nm excitation being used to generate fluorescence of bound PPE-DABCO. No fluorescent signal is observed in the absence of PPE-DABCO (G, H).