TABLE 1 .
Description and treatment of black-capped chickadees, red-breasted nuthatches, and Northwestern crows sampled
| Species and specimena | Yr | AKD status | Poecivirus present | Beak length (mm) | Beak appearance, histopathology |
NGSb | Sample processing notes | ||
|---|---|---|---|---|---|---|---|---|---|
| Elongated | Crossed | Normal, cellular-level hyperkeratosis | |||||||
| BCCH | |||||||||
| 160 | 2001 | Affected | Yes | 9.3 | X | Yes | Full Sanger sequencing of ORF | ||
| 263 | 2001 | Affected | Yes | 9.3 | X | Yes | |||
| 394 | 2001 | Affected | Yes | 9.4 | X | Yes | Full Sanger sequencing of ORF | ||
| 449 | 2002 | Affected | Yes | 9.5 | X | Yes | Full Sanger sequencing of ORF | ||
| 451 | 2002 | Affected | Yes | 11 | X | Yes | |||
| 478 | 2003 | Affected | Yes | 21.1 | X | X | Yes | ||
| 498 | 2004 | Affected | Yes | 40.3 | X | X | Yes | Full Sanger sequencing of ORF | |
| 600 | 2006 | Affected | Yes | 8.1 | X | Yes | |||
| 738 | 2010 | Affected | Yes | 8 | X | Full Sanger sequencing of ORF | |||
| 953 | 2014 | Affected | Yes | 25.1 | X | X | Stored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers | ||
| 954 | 2014 | Affected | Yes | 25.1 | X | Stored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers | |||
| 955 | 2014 | Affected | Yes | 23.1 | X | X | Poecivirus detection using virus-specific primers | ||
| 956 | 2014 | Affected | Yes | 8.7 | X | Stored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers | |||
| 967 | 2015 | Affected | Yes | 19.6 | X | X | Stored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers | ||
| 968 | 2015 | Affected | Yes | 9.5 | X | X | Stored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers | ||
| 970 | 2015 | Affected | Yes | 8.8 | X | Stored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers | |||
| 971 | 2015 | Affected | Yes | 18.7 | X | X | Stored at 4°C overnight prior to processing for poecivirus detection using virus-specific primers | ||
| 972 | 2015 | Affected | Yes | 15.7 | X | X | Poecivirus detection using virus-specific primers | ||
| 973 | 2015 | Affected | Yes | 9.0 | X | X | Poecivirus detection using virus-specific primers | ||
| 183 | 1995 | Unaffected | No | 7.3 | Poecivirus detection using virus-specific primers | ||||
| 389 | 2001 | Unaffected | No | 7 | Poecivirus detection using virus-specific primers | ||||
| 392 | 2001 | Unaffected | No | 7.3 | Poecivirus detection using virus-specific primers | ||||
| 401 | 2001 | Unaffected | No | 7.3 | Poecivirus detection using virus-specific primers | ||||
| 460 | 2003 | Unaffected | No | 7.3 | Poecivirus detection using virus-specific primers | ||||
| 473 | 2003 | Unaffected | No | 7.3 | Poecivirus detection using virus-specific primers | ||||
| 596 | 2007 | Unaffected | Yes | 7 | Full Sanger sequencing of ORF | ||||
| 601 | 2008 | Unaffected | Yes | 7.4 | Full Sanger sequencing of ORF | ||||
| 739 | 2010 | Unaffected | No | 7.3 | Poecivirus detection using virus-specific primers | ||||
| RBNH | |||||||||
| 12 | 1999 | Affected | Yes | 12.1 | X | X | Poecivirus detection using virus-specific primers | ||
| 929 | 2012 | Affected | Yes | 29.6 | X | X | Poecivirus detection using virus-specific primers | ||
| NOCR | |||||||||
| 674 | 2005 | Affected | Yes | 36.3 | X | Poecivirus detection using virus-specific primers | |||
| 848 | 2007 | Affected | Yes | 33.1 | X | X | Poecivirus detection using virus-specific primers | ||
a
BCCH, black-capped chickadees; RBNH, red-breasted nuthatches; NOCR, northwestern crows.
b
NGS, next-generation sequencing.