Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Plasmids used in this study
| Designation | Relevant characteristic(s) | Source or reference |
|---|---|---|
| pCR2.1 | Cloning vector | Invitrogen |
| pGP704 | ori(R6K) mobRP4 (Carbr) | 60 |
| pEP185.2 | ori(R6K) mobRP4 (Cmr) | 61 |
| pFR6 | tsr open reading frame and promoter cloned into pWSK29 | 13 |
| pSW127 | ′invA′ gene fragment cloned into pGP704, insertion of pSW127 into IR715 chromosome invA gene | 30 |
