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. 2016 Jul 12;7(4):e01006-16. doi: 10.1128/mBio.01006-16

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Copyright © 2016 Paredes et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — S. poulsonii and wasps compete for hemolymph lipids. (A) Quantification of hemolymphatic DAGs in D. melanogaster flies with or without wasp infestation and harboring (+) or not harboring (−) S. poulsonii (Sp). *, P = 0.0041; t = 3.268; df = 19; **, P = 0.0450; t = 2; df = 18; **, P = 0.0020; t = 3.612; df = 18; using unpaired Student’s t test. (B) Absolute quantification of S. poulsonii titers by qPCR. **, P = 0.00286; two-way ANOVA; see Table S1 in the supplemental material for details. (C) Fly survival after Lpp knockdown mediated by the activation of UAS-iLpp in the fat body using a specific thermosensitive driver (C564-Gal4^TS, C564^TS>). UAS-iLpp in the absence of driver (UAS-iLpp/+) and Oregon-R (OR^R) flies were used as negative controls. (C) D. melanogaster (D.) dead larvae and pupae, emerging fly adults, and wasp adults. Results are percentages of a minimum of 270 D. melanogaster larvae. ***, from left to right, respectively: P < 2.2 × 10⁻¹⁶; chi-square = 153.96; df = 3; P < 2.2 × 10⁻¹⁶; chi-square = 620.75; df = 3; P < 2.2 × 10⁻¹⁶; chi-square = 84.458; df = 3; using Pearson’s chi-square test. (A to C) Results are means ± SEM from at least three independent experiments.