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. 2016 Jun 22;5(7):962–969. doi: 10.1242/bio.018572

Fig. 5.

Fig. 5.

MALLS results for MICU2-NΔ84-CΔ28 and EF-hand mutations. (A,C,E) MALLS results for MICU2-NΔ84-CΔ28 and EF-hand mutations in Ca2+-free (2 mM EGTA) condition. (B,D,F) MALLS results for MICU2-NΔ84-CΔ28 and EF-hand mutations in Ca2+-bound (5 mM CaCl2) condition. Ca2+ could change MICU2-NΔ84-CΔ28 and MICU2-NΔ84-CΔ28EF2mut from a monomer to a dimer which is not the case for MICU2-NΔ84-CΔ28EF1mut. Therefore, the conformational change would take place in parallel with Ca2+ binding with the first EF-hand. The red line indicates the laser light scattering data and the blue line indicates the differential refractive index data.