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. 2016 Jul 15;129(14):2732–2743. doi: 10.1242/jcs.184150

Fig. 2.

Fig. 2.

FLAG-tagged and GFP-tagged p.S143P lamin A form intranuclear aggregates. (A) Fibroblasts from a healthy donor were transduced with lentiviral vectors encoding either FLAG-tagged WT lamin A or p.S143P lamin A and stained with anti-FLAG and anti-lamin A (LA) antibodies, and DAPI. Single mid-plane confocal sections and maximum projections are shown. Scale bar: 10 μm. (B) HeLa cells were transfected with either GFP–WT-LA or GFP–p.S143P-LA and stained with anti-lamin B1 (LB1) antibodies and DAPI. Single mid-plane confocal sections and maximum projections are shown. Scale bar: 10 μm. (C,D) TEM analysis of HeLa cells transfected with either FLAG-tagged WT lamin A or p.S143P lamin A (C) or GFP-tagged WT lamin A or p.S143P lamin A (D) showing electron-dense nuclear aggregates at the lamina region in mutant-transfected cells. Scale bars: 5 µm (upper left panel in C), 2 µm (other panels in C,D).