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. 2016 Jul 15;129(14):2804–2816. doi: 10.1242/jcs.188045

Fig. 3.

Fig. 3.

SNX9 directly binds TKS5. (A,B) GFP pulldown assays of TKS5–GFP with endogenous SNX9 (A) or GFP–SNX9 with endogenous TKS5 (B), using MDA-MB-231 cell lysates. (C) Scheme of WT and deletion mutants of SNX9 used in E. (D) Scheme of WT and deletion mutants of SNX9 used in F. (E) HA pulldown of HA-tagged WT or mutant SNX9 using lysates from MDA-MB-231 cells transiently co-expressing exogenous SNX9 with TKS5–GFP. (F) GST pulldown assay of bacterially expressed and purified His–TKS5 using GST–WT-SNX9 or its deletion mutants as bait. Asterisks indicate GST–SNX9 proteins.