Skip to main content
. 2016 Jul 15;129(14):2804–2816. doi: 10.1242/jcs.188045

Fig. 5.

Fig. 5.

SNX9 regulates MT1-MMP internalization and invadopodia activity. (A) 231-CTR or 231-siSNX9 cells were plated on green fluorescent gelatin and stained for F-actin (phalloidin, in red). Dark areas on the green channel correspond to matrix degradation. (B) Corresponding quantification of invadopodia-dependent matrix degradation (see Materials and Methods). n=3, ***P<0.005 (au=arbitrary unit). (C) 231-CTR or 231-siSNX9 cells were incubated with anti-MT1-MMP–Alexa-Fluor-448 antibody at 37°C for 20 or 60 min to allow the internalization of the antibody. The contours of cells are outlined in yellow. Arrows point to vacuoles containing MT1-MMP. 231-siSNX9 contain fewer vacuoles compared with 231-siCTR cells. (D) Corresponding quantification of anti-MT1-MMP antibody internalization. n=3; *P≤0.05. (E) TIRF microscopy image of 231-CTR or 231-siSNX9 stained using red phalloidin and with anti-MT1-MMP–Alexa-Fluor-448 antibody. Insets show enlargement of yellow boxes. Results on bar charts are presented as mean±s.e.m. Statistical significance was evaluated using one-tailed Mann–Whitney test. Scale bars: 20 μm in A; 10 μm in C; 10 μm, insets 2 μm in E. Arrows in E indicate colocalization of actin with MT1-MMP at invadopodia.