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. 2016 Jul 1;129(13):2501–2513. doi: 10.1242/jcs.186338

Fig. 6.

Fig. 6.

CEP295 overexpression induces extra-long microtubule-based filaments with low efficiency. (A) G-CEP295R-inducible cells were treated with doxycycline for 2 days (i-iii) or 4 days (ii), and analyzed by confocal fluorescence microscopy using indicated antibodies. Percentages of cells with elongated centrioles and centriole length were quantified. Centriole length labeled by acetylated tubulin (Ace-tub) >0.5 µm was counted as elongated centriole. Error bars in ii represent the mean±s.e.m. from three independent experiments (n=100 cells/experiment). Error bars in iii show the mean±s.d. (B–D) U2OS and G-CEP295R-inducible cells were immunostained with antibodies against Ace-tub and other centriolar proteins including POC5 (B), centrin2 (CEN2; C), and CEP164 (a mother distal appendage marker; D). Schematics on right of D indicate centrioles (black rectangles) and positions of CEP164 (red squares). MC, mother centriole; DC, daughter centriole. (E) The quantitation of percentage of CEP164 signal at elongated centrioles in G-CEP295R-inducible cells. Error bars represent the mean±s.e.m. from two independent experiments. The total number n as indicated.