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. 2016 Jul 1;129(13):2651–2659. doi: 10.1242/jcs.189076

Fig. 2.

Fig. 2.

Cad99C is transported apically along microtubules that are polarised by Patronin and Shot. (A) Control egg chamber stained for Cad99C, Rab11, DAPI and Tubulin to show polarised microtubules. (B) Overexpression of Katanin60 causes microtubules to depolymerise, resulting in loss of Rab11 and Cad99C polarisation (arrows). Control egg chambers (C) or egg chambers treated with colchicine (D) to depolymerise microtubules; Rab11 polarisation is lost upon colchicine treatment. Expression of Patronin–GFP (E) and Shot–GFP (F) shows both proteins localise apically. Expression of Patronin RNAi (G) or mutation of shot (GFP-positive clone) (H) causes depolarisation microtubules, affecting Rab11 localisation and Cad99C protein levels. (I–L) Combined perturbation of Patronin and Shot (GFP-positive clone) results in loss of Cad99C from the apical membrane and causes severe defects in microtubule polarisation. Loss of polarised microtubules results in the mislocalisation of nuclei in these mutants, which gives the impression of multilayering of the follicle cells (arrows in I).