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. 2016 Jul 1;143(13):2398–2409. doi: 10.1242/dev.130658

Fig. 3.

Fig. 3.

Impaired astrocytic differentiation, but normal neuronal differentiation, in YAP-deficient NSCs. (A) Immunostaining analysis of Tuj1 (green) in neurons differentiated from WT and YAP-deficient neurospheres (by incubating with neurobasal medium plus 2% B27). (B) Western blot analysis of Tuj1 in neurons differentiated from WT and YAP-deficient neurospheres. (C) Quantitative analysis of western blot data (n=3 per group, normalized to the WT group) as shown in B. (D) Immunostaining analysis of GFAP (green) in astrocytes differentiated from WT and YAP-deficient neurospheres (by incubating with DMEM plus 10% FBS). (E) Quantitative analysis of the relative GFAP intensity (normalized to the WT group, n=6) as shown in D. (F) Western blot analysis of GFAP expression in astrocytes differentiated from WT and YAP-deficient neurospheres. (G) Quantitative analysis of western blot data (n=4 per group, normalized to the WT group) as shown in F. Data are mean±s.e.m. **P<0.01, compared with control group, Student's t-test. Scale bars: 20 μm.