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. 2016 Jul 1;143(13):2344–2355. doi: 10.1242/dev.134577

Fig. 10.

Fig. 10.

Detection of PNA binding to frontal sections of developing palatal shelves. (A-F′) Frontal sections through the anterior (A-B′), middle (C-D′) and posterior (E-F′) regions of the palatal shelves of control (A,A′,C,C′,E,E′; n=6) and homozygous mutant (B,B′,D,D′,F,F′; n=6) embryos were stained with fluorescein-conjugated PNA with (A-F) or without (A′-F′) pretreatment with neuraminidase (NEU). Arrows point to nasal side of the palatal shelf in each panel. (G,G′) Quantification of mean fluorescence intensity across PNA-stained palatal shelf regions from three pairs of control and mutant littermates either with (G) or without (G′) NEU pretreatment. Error bars represent s.d.; n.s., difference not statistically significant (P>0.05); *P<0.05; ***P<0.001.