Fig. 5.
APC2-dependent isoform changes are due to phosphorylation and other PTMs. (A) Model for the PTM responsible for Dp1 isoforms. (A′,A″) Dp1 isoforms are affected by phosphorylation, as the left isoform collapses to the right isoform after λPP treatment for both wild-type and Apc2g10 embryo lysates. (B) Model for the PTM(s) responsible for ApepP isoforms. (B′,B″) ApepP isoforms were not affected by λPP treatment, as there were no isoform shifts observed after phosphatase treatment. Difference-protein isoforms (L, left; M, middle; R, right) are circled.