Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 15;143(14):2603–2615. doi: 10.1242/dev.132969

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 4. — ephb4b genetic mutants exhibit defects in DFC aggregation and laterality. (A) Illustration of the ephb4b mutant allele (Mt) generated by the Cas9 knockout approach. (B) ephb4b expression pattern in different types of embryos at 75% ES. The siblings were genotyped after being photographed and the proportion of embryos exhibiting the representative pattern is indicated. (C) Morphology of live WT and mutant siblings at 28 hpf. (D-F) Heart jogging was detected by probing cmlc2 expression at 28 hpf (D) and quantified (E). ns, not significant; **P<0.01. After in situ hybridization, each embryo was genotyped by PCR as shown in F. (G) DFC aggregation was examined by probing sox17 expression at 75% ES. Shown are representative DFC patterns in mutants only. The DFC region is enlarged in the lower panels. (H) Representative genotyping results for DFCs-III embryos. (I) Embryos at the bud stage were immunostained for GFP expression and observed by confocal microscopy. The AP axis is specified.