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Detection of IL-4 in spleens of immunized mice by ELISPOT. (a) Vaccines given via i.m. route; (b) vaccines given via i.p. route. P values less than 0.05 were considered significant and, when represented by asterisks, are shown as follows: ∗0.05 ≥ P > 0.01; ∗∗0.01 ≥ P > 0.001; ∗∗∗0.001 ≥ P > 0.0001; ∗∗∗∗ P ≤ 0.0001.
