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Validation of differential expression of mitochondrial uncoupling protein-2 and SOD2 in RC77N/E and RC77T/E in cell lysates. Normalized total protein lysates from the two cell lines were subjected to Western blot analysis to detect UCP2 (a) and SOD2 (b). All the samples were normalized with respect to GAPDH, which was included as a loading control. The corresponding ImageJ and normalized densitometry comparisons are shown in (c) and (d), respectively.
