Table 1.

Summary of ruthenylated antibody analysis by HPSEC. Ruthenylated antibody was stored at 1 mg/mL in PBS) or in HSB. Each preparation was subjected to multiple freeze-thaw (F/T) cycles by cycling between −80 ± 10°C (approximately 16 hours) and 5 ± 3°C (approximately 8 hours) prior to analysis by HPSEC.

Retention time [min]	Molecular weight [kDa]	Structure designation	% peak area
			0 F/T cycles		1 F/T cycle		3 F/T cycles		5 F/T cycles
			PBS	HSB	PBS	HSB	PBS	HSB	PBS	HSB
11.45	~1900	Unordered multimer	0.09	0.0	0.22	0.0	0.44	0.0	0.31	0.0
12.45	~360	Dimers, trimers	2.6	0.2	2.9	0.2	3.6	0.2	2.8	0.2
14.89	~150	Monomer	97.3	99.7	96.9	99.7	95.9	99.6	96.9	99.7