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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2016 May 20;1859(8):1043–1055. doi: 10.1016/j.bbagrm.2016.05.009

Figure 2. Chromatin-modifying enzymes associated with the Runx2/p57 promoter in developing hippocampus.

Figure 2

Chromatin immunoprecipitation (ChIP) assays were performed on samples obtained from E18, P10, P30 and P>90 rat hippocampus and incubated with antibodies against Ezh2 (A), Ezh1 (B), PRMT5 (C), Wdr5 (D), SUV39H1 (E), and HP1 (F). The precipitated DNA was quantified using specific primers against the indicated region of the Runx2/p57 promoter. Primers for PSD95 promoter region were used as positive controls. Results are shown as Input (%) ± SD of at least three independent experiments. Normal IgG was used as specificity control. *: P < 0.05, **: P < 0.01, ***: P < 0.001, with respect to E18 (ANOVA). For the right panel of D, ***: P < 0.001, with respect to IgG (Student's t-test).