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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2016 May 20;1859(8):1043–1055. doi: 10.1016/j.bbagrm.2016.05.009

Figure 5. Knockdown of PRMT5 does not alter repression of the Runx2/p57 gene in primary hippocampal cells.

Figure 5

Mature hippocampal cells were infected at 17DIV with a lentivirus carrying a shRNA against PRMT5 expression. (A): Total RNA samples were obtained 96h later to confirm the PRMT5 silencing by qRT-PCR (left panel). Results were normalized and expressed as described in Figure 3. PRMT5 knockdown was also confirmed by Western blot using specific antibodies (right panel). Detection of TFIIB was used to control for equal protein loading. (B): ChIP experiments were performed on samples using specific antibodies against H3K27me3. Results are expressed as Input (%) ± SD of at least three independent experiments. Normal IgG was used as specificity control. (C): Potential changes in mRNA expression of the indicated osteoblastic genes were determined by qRT-PCR. ***: P < 0.001, with respect to the corresponding control (empty vector) value (Student's t-test).