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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Biopolymers. 2016 Oct;105(10):715–724. doi: 10.1002/bip.22874

Figure 1.

Figure 1

Objective-lens based TIRF microscopy to detect specific interactions between flurophore-free α-Syn monomers on the cover slip and fluorophore-labeled counterparts in solution. The experiments are carried out at acidic pH. A sudden increase and abrupt drop of fluorescence intensity are unambiguously ascribed to the association and dissociation of a dimer within the evanescent field of TIRF, respectively. The short dwell time between association and dissociation are interpreted as a lifetime of a dimer.