Fig. 5.
APC profiling of SPL, MLN after prenatal antibiotic exposure. Cells were gated on CD11b+ or CD11c+ cells and CD80 and CD86 expression was examined. (A) CD80 and CD86 expressing CD11b+ cells. Representative FACS plots of CD11b+CD86+ cells from MLN. (B) CD80 and CD86 expressing CD11c+ cells. (C) Total lymphocytes were isolated and stimulated with LPS or CPG and tested for IFNγ-expressing CD11b+ and CD11c+ cells. (D) IFNγ expressing CD11b+ and CD11c+ cells from prenatally antibiotic-treated NOD. *: P<0.05, **: P<0.01, ***: P<0.001 (Student’s t-test).
