. Author manuscript; available in PMC: 2018 Mar 1.

Published in final edited form as: Neurotoxicology. 2016 Jan 23;59:164–174. doi: 10.1016/j.neuro.2016.01.002

Table 4.

Comparison of transsulfuration metabolites in plasma of control and TCE-treated mice. Results represent the mean (SD).

Plasma thiols	0 mg/ml TCE	0.01 mg/ml TCE	0.1 mg/ml TCE
GSH (μM)^f	2.52 (0.17)	2.14 (0.33)	1.37 (0.50)^***
GSSG (μM)	0.63 (0.12)	0.91 (0.41)	1.42 (0.65)^*
GSH/GSSG	4.12 (0.75)	2.77 (1.34)	1.44 (1.35)^**
% oxidized GSH	33.05 (3.84)	44.64 (12.27)	62.35 (18.25)^**
Total GSH	3.77 (0.34)	3.96 (0.64)	4.20 (0.99)

CyS (μM)^f	17.33 (2.71)	20.90 (7.35)	15.43 (2.15)
CySSCy (μM)	21.70 (2.59)	27.67 (6.31)	29.02 (3.52)^*
Cys/CySSCy	0.80 (0.87)	0.74 (0.12)	0.54 (0.09)^**
Total CyS	60.73 (7.37)	76.23 (19.7)⁺	73.46 (7.77)^*

Total glutathione (GSH) and cysteine (CyS) was calculated as described in Materials and Methods.

Reflects free or unbound thiols remaining after protein precipitation. Results are statistically different from results obtained from controls

p≤0.05,

^**

p≤0.005,

^***

p≤0.0005.

⁺

Variance ratios are statistically different (p=0.02).