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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Addict Biol. 2016 Jan 24;22(3):616–628. doi: 10.1111/adb.12353

Figure 5. The decrease in the tonic GABAAR current following adolescent ethanol exposure reflects attenuation of current mediated by δ-subunit containing receptors.

Figure 5

A) Representative trace showing the effect of bath application of the selective δ-GABAAR agonist THIP. B) Representative all-point histogram and Gaussian function that were used to measure the effect of THIP as a change in the Ihold for each individual cell. C) THIP-mediated change in the Ihold in PrL-C slices obtained from control and AIE exposed rats at different developmental ages. In slices obtained prior to initiation of AIE exposure (PD28, n = 5 cells/group), shortly after the last cycle of AIE exposure (PD45, n = 5 cells/group), and in early adulthood (PD60, n = 7 cells/group) there were no differences in THIP modulation of holding current. However, in slices from adult rats, there was a significant reduction in the THIP mediated current (n = 9-10 cells/group, *p = 0.04). D) AIE exposure significantly attenuated THIP-mediated current in layer V pyramidal neurons from adult female rats (n = 6-9 cells/group **p < 0.01).