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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Addict Biol. 2016 Jan 24;22(3):616–628. doi: 10.1111/adb.12353

Figure 8. Adolescent ethanol exposure has no effect on expression of GABAAR subunits that contribute to the tonic current.

Figure 8

A) Protein expression of GABAAR subunit in the PrL-C of AIE exposed adult rats measured in total tissue extracts (n = 8-9 rats/group). B) Surface expression of δ-GABAARs using BS3 cross-linking of surface membrane proteins in acute slices. Shown on the left is loss of the δ-subunit and total protein upon detergent permeabilization of the slice in the presence and absence of the cross-linking reagent BS3. Shown on the right is quantification of δ-subunit surface expression expressed as the difference between the non-cross-linked intracellular and the total subunit expression (n = 13-14 rats/group). C) Representative image from an Air exposed control rat of immunohistochemical labeling for the δ-subunit showing differential expression in superficial and deep layers of the cortex. D) Quantification of the boxed area in C revealed significantly greater δ-subunit expression in superficial versus deep layers of the PrL-C (p < 0.001) but a significant effect of AIE exposure was not observed.