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. 2016 Jul 25;7:217. doi: 10.3389/fphar.2016.00217

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Copyright © 2016 Dan, Liu, Wong and Ng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ganoderma lucidum ribonuclease induced cell cycle arrest at G1 phase. (A) HT29 and HCT116 cells were treated with different concentrations of GLR for 24 h. Cells were fixed in ice-cold ethanol and stained with PI for flow cytometric analysis. (B) Cells were seeded on the coverslips in a 24-well plate and cultured in the presence of 1 μM GLR and 10 μM EdU for 24 h. Cells were observed by using a confocal microscope after the labeling of Alexa Fluor^® 555. The percentage of EdU positive cells was displayed in the right panel (n = 3). ^∗∗P < 0.01, ^∗∗∗P < 0.001 versus respective control groups. (C) Western blotting analysis of cyclin D1 expression in HT29 (C) and HCT116 cells (D) was conducted after cells had been treated with indicated concentrations of GLR for 24 or 48 h. GAPDH was used as an internal control.