FIGURE 5.

Ganoderma lucidum ribonuclease induced cell apoptosis in colorectal cancer cells. (A) Cells exposed to the indicated concentrations of GLR for 24 or 48 h were harvested and subsequently stained with Annexin V/PI solution. Cells were then sorted by using flow cytometry. (B) Cells were treated with GLR at the indicated concentrations for 24 h, and nuclear morphological changes and caspase activation were, respectively, examined by staining with Hoechst 33342 and image-iT LIVE Green Poly Caspases staining reagent. Bars, 200 μm. Expression of Apaf-1, Bax, caspase-9, and PARP in HT29 cells (C) and HCT116 cells (D) was analyzed by western blotting after cells had been treated with different concentrations of GLR for 24 or 48 h. GAPDH was used as an internal control.