FIGURE 6.

Ganoderma lucidum ribonuclease suppressed the activation of cell autophagy in colorectal cancer cells. (A) HT29 cells and HCT116 cells were treated with the indicated concentrations of GLR for different durations before the protein expression levels of P62, LC3-I, and LC3-II were determined by western blotting. GAPDH was used as an internal control. (B) Subcellular expression and distributions of pEGFP-LC3. Colorectal cancer cells were transfected with the pEGFP-LC3 vectors in OPTI-MEM I Reduced Serum Medium for 6 h. After treatment with 2 μM GLR for 48 h, the fluorescence signals of cells were acquired by confocal microscopy. (C) Ultrastructures of HT29 cells and HCT116 cells treated with or without GLR. Cells were cultured with medium containing 0 or 1 μM GLR for 24 h before preparation for TEM sections. N, nucleus; M, mitochondrion; AVs, autophagic vacuoles.