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. 2016 Jul 25;9:58. doi: 10.3389/fnmol.2016.00058

Figure 4.

Figure 4

Cellular localization of OPN in blue LED-induced RD retinas. Confocal micrographs taken from vertical sections of blue LED-induced RD retinas processed for OPN (green) and Iba1 (red) immunoreactivity. (A–F) A representative RD retina at 72 h after blue LED exposure. OPN was observed in Iba1-labeled microglia (arrows) in the ONL and OPL. INL, inner nuclear layer; IPL, inner plexiform layer; IS/OS, inner segment and outer segment; ONL, outer nuclear layer; OPL, outer plexiform layer; RPE, retinal pigment epithelium. A region including two OPN-labeled microglia in (A–C) are magnified in (D–F), respectively. OPN was localized in the processes of Iba1-labeled microglial cells. (G–J) A representative RD retina at 72 h after blue LED exposure. Iba1-labeled cells were observed in the INL, IPL, and subretinal space. OPN was observed in Iba1-labeled microglia of the ONL and microglia/macrophages of the subretinal space (arrow), while OPN expression was not observed in Iba1-labeled microglia of the INL or IPL. Two microglia/macrophages (arrows) showing Iba1 and OPN co-labeling are magnified in (J). (K) A representative RD retina at 120 h after blue LED exposure. Several Iba1-labeled microglia/macrophages were still observed in the ONL and subretinal space, while minimal OPN-labeling was observed in the ONL and subretinal space.