Table 3.

qRT-PCR validation of genes (n = 13) identified by microarray analysis as differentially expressed in the spleen of beef steers with variation for feed intake and weight gain.

	PPAP2C		CREM		DNAJC5		BAG3		DNAJB1
	qRT-PCRa	Microarrayb	qRT-PCR	Microarray	qRT-PCR*c	Microarray	qRT-PCR**d	Microarray	qRT-PCR**	Microarray
High gain-high intake	0.064	7.392	0.09	8.565	−0.0515	10.624	−0.145	9.749	0.524	12.184
High gain-low intake	−0.205	7.331	−0.0991	8.258	−0.0371	10.609	−0.312	9.474	0.563	12.210
Low gain-low intake	−0.331	7.106	−0.0142	9.270	0.0322	9.999	0.338	10.929	0.975	14.027
Low gain-high intake	−0.209	6.974	−0.0238	8.379	0.0441	10.097	−0.196	9.237	0.525	12.255
SEe	0.0152	0.0708	0.097	0.106	0.028	0.172	0.102	0.294	0.082	0.430
	HADHB		SCN4B		GBP7		HSPH1
	qRT-PCR	Microarray	qRT-PCR*	Microarray	qRT-PCR*	Microarray	qRT-PCR**	Microarray
High gain-high intake	−0.0211	11.741	−0.336	7.712	−0.102	9.982	−0.0486	12.823
High gain-low intake	−0.055	11.333	−0.378	7.398	0.0171	11.177	−0.172	12.443
Low gain-low intake	−0.0841	11.329	0.127	7.946	0.0443	10.324	0.409	15.450
Low gain-high intake	−0.0789	11.371	−0.173	7.638	−0.00681	10.111	−0.175	12.164
SE	0.053	0.0541	0.134	0.0613	0.0707	0.235	0.117	0.448
	KCNMA1		LPIN1		SIRT6		TSHZ2
	qRT-PCR	Microarray	qRT-PCR	Microarray	qRT-PCR	Microarray	qRT-PCR	Microarray
High gain-high intake	0.15	7.806	−0.0992	9.393	0.11	9.313	0.102	9.651
High gain-low intake	−0.0231	7.218	−0.118	9.310	0.0874	9.593	−0.0294	9.347
Low gain-low intake	−0.0193	7.553	−0.121	10.114	0.0579	9.250	−0.0895	8.737
Low gain-high intake	0.23	8.201	−0.0345	9.404	0.0931	9.500	0.0511	9.733
SE	0.129	0.108	0.133	0.167	0.0862	0.0567	0.0673	0.117

^aLog transformed 2^−ddCT values from qRT-PCR.

^bRelative expression from microarray data log 2 transformed.

^c*P ≤ 0.01.

^d**P ≤ 0.05.

^eStandard errors given for qRT-PCR and microarray analyses.