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. 2016 Jul 25;7:1094. doi: 10.3389/fpls.2016.01094

FIGURE 2.

FIGURE 2

Putative structural genes involved in the biosynthesis of terpenoid backbone in Anemone flaccida. A flow diagram of biosynthetic pathway of triterpenoid in A. flaccida. The chemicals in the MVA pathway are shown in dark boxes, while green boxes are for MEP pathway. The words in blue are enzymes catalyze the reaction. The transcript numbers are shown in red brackets. Abbreviations used are: AACT, acetyl CoA C-acetyltransferase (EC 2.3.1.9); HMGS, 3-hydroxy-3-methylglutaryl CoA synthase (EC 4.1.3.5); HMGR, 3-hydroxy-3-methylglutaryl CoA reductase (EC 1.1.1.88); MK, mevalonate kinase (EC 2.7.1.36); PMK, phosphomevalonate kinase (EC 2.7.4.2); MDC, mevalonate-5-pyrophosphate decarboxylase (EC 4.1.1.33); IPPI, isopentenyl diphosphate isomerase (EC 5.3.3.2); GPPS, geranyl diphosphate synthase (EC 2.5.1.1); FPPS, farnesyl diphosphate synthase (EC 2.5.1.10); SS, squalene synthase (EC 2.5.1.21); SE, squalene epoxidase (EC 1.14.99.7); β-AS, β-amyrin synthase (EC 5.4.99.–); PDMO, cytochrome P450-dependent monooxygenases; GT, glycosyltransferases; DXPS, 1-deoxy-D-xylulose-5-phosphate synthase (EC 2.2.1.7); DXR, 1-deoxy-D-xylulose-5-phosphate reductoisomerase (EC 1.1.1.267); MCT, 2-C-methyl-D-erythritol 4-phosphate cytidylyl transferase (EC 2.7.7.60); CMK, 4-(cytidine 5’-diphospho)-2-C-methyl-D-erythritol kinase (EC 2.7.1.148); MECPS, 2-C-methyl-D-erythritol-2, 4-cyclodiphosphate synthase (EC 4.6.1.12); HDS, 4-hydroxy-3-methyl but-2-(E)-enyl diphosphate synthase (EC 1.17.7.1); HDR, 4-hydroxy-3-methyl but-2-(E)-enyl diphosphate reductase (EC 1.17.1.2).