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. 2016 Jul 25;6:30421. doi: 10.1038/srep30421

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Copyright © 2016, The Author(s)

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pGL3−1826 or mutant pGL3−1826 and STAT3 overexpression plasmids were co-transfected into LS174T cells, and hTFF3 was added at 24 h post-transfection, followed by continuous culture for 24 h. (A) Detection of relative fluorescence intensity. (B) Real-time analysis of STAT3 and hTFF3 mRNA levels. (C,D) Western blot analysis of hTFF3 protein levels. Data are presented mean ± S.D. (*P < 0.05 compared with the sample expressing pCDNA3.1).