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. 2016 Jul 25;6:30189. doi: 10.1038/srep30189

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Copyright © 2016, The Author(s)

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(A) Investigation of the suitable time window of 10 ng/ml CA-4 treatment. The phenotypes of embryo dosed at gastrula, segmentation, pharyngula periods were analyzed. The experiments of CA-4 treatment during each time window were repeated in triplicate at least. (B,C) Percentage of phenotype induced by 1–100 ng/ml CA-4 treatment at 8 hpf, analyzed at 36 hpf (B) and 60 hpf (C), respectively. The experiments of CA-4 treatment at each concentration were repeated in triplicate. The percentage of Dead, Malformed, Tardive and Normal was displayed in Grey, Pink, Orange and Green columns, respectively.