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. 2016 Jul 25;6:30189. doi: 10.1038/srep30189

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Copyright © 2016, The Author(s)

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(A) Whole-mount in situ hybridization analysis of notch1a expression in control embryo and CA-4-treated embryo. Red arrowhead indicates neural tube. (B) RT-PCR analysis of notch1a expression in control embryo and CA-4-treated embryo. (C) Real-time PCR analysis of notch1a expression in control embryo and CA-4-treated embryo. (D) Confocal analysis of CA-4-treated Tg (huc:EGFP) embryo. (E) Statistical analyses of EGFP positive cells in central nervous system of control, CA-4-treated, CA-4 and DAPT treated, and CA-4 treated p53 knockdown embryo. (n = 6), ***P < 0.001.