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. 2016 Jul 25;6:30085. doi: 10.1038/srep30085

Figure 3. Norepinephrine decreased aggrecan expression, but increased expression of MMP-3, MMP-13 and RANKL.

Figure 3

(A) Real-time PCR of the expression of aggrecan, type II collagen (Col2), type X collagen (ColX), MMP-3, MMP-9, MMP-13, RANKL, OPG and RANKL/OPG ratio by chondrocytes after NE stimulation (N = 5). The chondrocytes were isolated from condylar cartilage of 6-week female rats, and were treated with NE for 1, 4 or 12 h at 10−6, 10−7 or 10−8 M, respectively. (B) Western blot of the expression of aggrecan, MMP-3, MMP-13 and RANKL by chondrocytes after NE stimulation (N = 3). The chondrocytes were treated with 10−8 M NE for 1, 4 or 12 h, and treated 1h by 10−6, 10−7 or 10−8 M of NE, respectively. *P < 0.05, **P < 0.01: vs vehicle-treated chondrocytes.