Figure 7. In vitro dissociation of the FXIIIA₂B₂ heterotetramer observed by gel filtration.

Gel filtration elution profiles for purified heterotetramer FXIIIA₂B_2, at various calcium & thrombin concentrations in local environment during in-vitro activation. The color key (with number) depicts the different treatment conditions during activation (number corresponds to the table on right). X-axis shows the retention volume of peak; y-axis shows the amount of protein in mAU. The table also details the retention time of the peaks corresponding to FXIIIA₂B_{2 (peak 1)} and FXIIIB_{2 (peak 2),} at different conditions, respectively. Protein identities eluted at peak maxima confirmed by mass spectrometry (Supplementary Figure S10).