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. 2016 Jul 25;6:30236. doi: 10.1038/srep30236

Figure 4. Microtubule dynamics during cell height change.

Figure 4

All the images are x-z views. Stains are anti-α-tubulin and DAPI. White arrowheads indicate the PE layers. Red arrowheads indicate the apical side of DP cells. Blue arrows indicate the intermediate cells. Green bars in (D–G) indicate the cell height of PE. (A–C) Time course of microtubule dynamics in wild type control wing discs. PE height starts to flatten at the early L3 stage (B, arrowhead). Microtubules are polarized to the apical side of the DP (C,D, red arrowheads) compared with the even distribution in PE (C,D, white arrowheads) and intermediate cells (C, blue arrows). (E) Microtubules are enhanced in the PE (white arrow) when sal is expressed. (F) lin expression induces similar microtubule changes upon sal expression. Note that the cell height and microtubule levels of the PE (E,F, white arrows) are similar to that of intermediate cells (C, blue arrows). (G) Co-expression of sal-RNAi and lin rescues PE height and microtubule levels similar to that noted in wild type.