Fig. 3.

Mb-IL-17A-promoted cell cycle progression verified by colcemid-induced metaphase arrest. (A) Cells were seeded and cultured for 30 h. Each group of cells was harvested at the indicated time points and stained with propidium iodide (PI). The proportion of cells in G1, S, and G2/M phases was measured by flow cytometry. (B) The 1 × 10⁶ cells of each transfectant type were treated with 0.1 μg/ml colcemid and the fraction of cells in G2/M phase at the indicated time points was measured by PI staining and flow cytometry. The differences between groups were analyzed using two-way ANOVA. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.