Fig. 4.

SPR activation in CG3542 neurons is insufficient for inducing egg-laying in virgin females. (A) Mating receptivity of virgin females of the indicated genotypes. The numbers inside each bar indicate n. Data are presented as mean percentages. Not significant (ns), p > 0.05 for comparisons against both controls (light gray bars); Fisher’s exact test. (B, D) Eggs laid per female of the indicated genotypes for 48 h post-mating. The numbers inside each bar indicate n. Data are presented as means ± SEM. ^**p < 0.01 and not significant (ns), p > 0.05 for comparisons against both controls (light gray bars); one-way ANOVA followed by Tukey’s test for multiple comparisons. (C, E, and F) Eggs laid per five virgin females of the indicated genotypes for 48 h. The numbers inside each bar indicate n. Data are presented as means ± SEM. ^***p < 0.001 and not significant (ns), p > 0.05 for comparisons against both controls (light gray bars); one-way ANOVA followed by Tukey’s test for multiple comparisons.