FIG 3.

Growth behavior in ACCM-D confirms C. burnetii amino acid auxotrophies. Amino acid prototrophies (A) and auxotrophies (B) of C. burnetii are indicated by growth characteristics in ACCM-D. Individual amino acids were omitted to generate ACCM-D amino acid dropout media. Media were inoculated with C. burnetii, and the fold change in bacterial replication was determined by qPCR to quantitate genome equivalents (GE) at 7 days (d7) and 14 days (d14) postinoculation. The results are expressed as the means of the results from three independent experiments, and the error bars indicate the standard deviations. Auxotrophy was scored as a fold change in GE/ml of <20 by 14 days postinoculation, while prototrophy was scored as a fold change in GE/ml of >1,000. A partial dependence on methionine was observed.