FIG 5.

Genetic transformation with pJB-CAT::argGH or pMiniTn7T-CAT::argGH complements C. burnetii arginine auxotrophy in ACCM-D lacking arginine but supplemented with citrulline. (A) Plasmid maps of pJB-CAT::argGH and pMiniTn7T-CAT::argGH containing argGH from L. pneumophila. (B) Growth of C. burnetii transformed with pJB-CAT::argGH or pMiniTn7T-CAT::argGH in ACCM-D, ACCM-D minus arginine, or ACCM-D minus arginine and supplemented with citrulline. Growth was quantified using qPCR to determine genome equivalents (GE) during a 14-day incubation. The results are expressed as the means of the results from three independent experiments, and the error bars indicate the standard deviations.