TABLE 1.
Carnivore coprosample collection and detection of host DNA and Toxocara sp. and E. multilocularis parasites by qPCRe
| Carnivore species | Sample code | Other animal(s) presentb |
Cq value |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| V. vulpes | C. lupus | F. catus | M. meles | M. foina | Toxocara | E. multilocularis | Toxo/Alea | Em/Alea | |||
| Vulpes vulpes | Em2736 | None | 25.8 | ND | ND | ND | ND | ND | 35.6 | 31.6 | 34.0 |
| Em2711a | None | 24.5 | ND | ND | ND | ND | ND | 26.3 | 32.1 | 34.1 | |
| Em2724 | None | 28.8 | ND | ND | ND | ND | ND | 39.5 | 33.4 | 34.1 | |
| Em2713 | None | 27.7 | 38.3 | 39.6c | ND | ND | ND | ND | 31.6 | 33.5 | |
| Em2603a | None | 18.1 | ND | ND | ND | ND | ND | 30.8 | 32.0 | 33.5 | |
| Em2416 | None | 17.9 | ND | ND | ND | ND | ND | 33.6 | 32.0 | 33.7 | |
| Em2714 | None | 25.5 | ND | ND | ND | ND | ND | 40.0 | 36.9 | 34.5 | |
| Em2738 | None | 25.4 | ND | ND | ND | ND | ND | 32.7 | 32.1 | 33.9 | |
| Em2692 | None | 24.9 | ND | ND | ND | ND | ND | 34.6 | 32.0 | 32.9 | |
| Em2888 | None | 21.4 | ND | ND | ND | ND | ND | ND | 32.5 | 33.4 | |
| Em2254 | None | 16.3 | ND | ND | ND | ND | ND | 31.5 | 33.6 | 35.0 | |
| Em2257 | None | 26.9 | ND | ND | ND | ND | ND | ND | 32.7 | 33.8 | |
| Em2562 | None | 17.2 | ND | ND | ND | ND | ND | 33.9 | 31.8 | 32.8 | |
| Canis lupus familiaris | YAYa | Cat/dog | ND | 22.0 | 33.3 | ND | ND | ND | ND | 32.9 | 35.0 |
| TIT | Cat/dog | ND | 20.2 | 37.7 | ND | ND | ND | ND | 34.3 | 36.2 | |
| IZIa | Cat | ND | 26.8 | ND | ND | ND | ND | ND | 35.3 | 37.8 | |
| 5108 | 2 dogs | ND | 23.3 | ND | ND | ND | ND | ND | 32.7 | 36.3 | |
| 5101 | None | ND | 21.6 | ND | ND | ND | ND | ND | 31.8 | 33.8 | |
| 5111 | Dog/cat | ND | 25.2 | ND | ND | ND | ND | ND | 34.4 | 37.6 | |
| 5065 | None | ND | 17.9 | ND | ND | ND | ND | ND | 32.2 | 34.4 | |
| 5077 | 3 dogs | ND | 20.2 | ND | ND | ND | ND | ND | 32.9 | 34.0 | |
| 5107 | 2 dogs | 42.0c | 24.0 | ND | ND | ND | ND | ND | 33.8 | 35.5 | |
| 5114 | None | ND | 20.9 | ND | ND | ND | ND | ND | 32.1 | 34.0 | |
| 5104 | None | ND | 22.0 | ND | ND | ND | ND | ND | 35.0 | 35.0 | |
| 5069 | None | ND | 25.4 | ND | ND | ND | ND | ND | 33.6 | 34.3 | |
| 5110 | None | ND | 26.2 | ND | ND | ND | ND | ND | 33.1 | 34.4 | |
| Canis lupus | 6850 | None | ND | 27.1 | ND | ND | ND | ND | ND | 33.9 | 35.2 |
| 6851 | None | ND | 25.1 | ND | ND | ND | ND | ND | 34.3 | 36.4 | |
| 6852a | None | ND | 21.4 | ND | ND | ND | ND | ND | 32.5 | 34.0 | |
| 6853 | None | ND | 27.9 | ND | ND | ND | ND | ND | 34.1 | 37.7 | |
| 6854 | None | ND | 26.5 | ND | ND | ND | ND | ND | 34.3 | 39.0 | |
| 6855 | None | ND | 27.8 | ND | ND | ND | ND | ND | 35.4 | 38.2 | |
| 6856 | None | ND | 26.4 | ND | ND | ND | ND | ND | 34.6 | 36.9 | |
| 6857 | None | ND | 28.7 | ND | ND | ND | ND | ND | 34.3 | 37.9 | |
| 6858d | None | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| 6865 | None | ND | 24.6 | ND | ND | ND | ND | ND | 32.9 | 34.2 | |
| 5670 | None | ND | 23.6 | ND | ND | ND | ND | ND | 33.1 | 34.9 | |
| 4861 | None | ND | 27.4 | ND | ND | ND | ND | ND | 35.7 | 37.9 | |
| 5667 | None | ND | 22.6 | ND | ND | ND | ND | ND | 32.6 | 34.1 | |
| Felis silvestris catus | POUa | Dog | ND | 33.6 | 25.3 | ND | ND | ND | ND | 36.6 | 37.8 |
| ESTa | None | ND | ND | 22.6 | ND | ND | ND | ND | 32.2 | 34.2 | |
| NOIa | Dog | ND | 34.4 | 23.5 | ND | ND | ND | ND | 33.3 | 34.2 | |
| ZIZa | None | ND | 34.1 | 22.4 | ND | ND | ND | ND | 33.3 | 35.5 | |
| GAM | ND | ND | 21.6 | ND | ND | ND | ND | 32.7 | 32.5 | ||
| PRI | ND | ND | 24.4 | ND | ND | 32.2 | ND | 33.7 | 35.6 | ||
| OSI | ND | 37.5c | 23.1 | ND | ND | 32.9 | ND | 31.6 | 33.0 | ||
| MIN | ND | ND | 21.7 | ND | ND | 32.1 | ND | 31.8 | 34.0 | ||
| EM5621 | None | ND | ND | 31.8 | ND | ND | ND | ND | 35.3 | 38.1 | |
| EM5212 | None | ND | ND | 27.9 | ND | ND | ND | ND | 35.4 | 37.8 | |
| EM5131 | None | ND | ND | 31.0 | ND | ND | ND | ND | 36.8 | 34.4 | |
| EM5191 | None | ND | ND | 30.4 | ND | ND | ND | ND | 35.2 | 35.0 | |
| EM5368 | None | ND | ND | 25.1 | ND | ND | ND | 40.2 | 35.5 | 37.6 | |
| EM5540 | Cat | ND | ND | 28.7 | ND | ND | ND | ND | 32.3 | 34.5 | |
| EM5536 | None | ND | ND | 21.0 | ND | ND | ND | ND | 34.1 | 36.5 | |
| EM5072 | Cat | ND | ND | 25.9 | ND | ND | ND | ND | 32.2 | 34.9 | |
| EM5018 | None | ND | 37.0c | 26.8 | ND | ND | ND | ND | 31.8 | 32.9 | |
| Meles meles | B1 | None | ND | ND | ND | 27.9 | ND | ND | ND | 31.7 | 33.7 |
| B2a | None | ND | ND | ND | 24.5 | ND | ND | ND | 35.6 | 34.8 | |
| B3d | None | ND | ND | ND | 32.4 | ND | ND | ND | 37.2 | ND | |
| B4 | None | ND | ND | ND | 23.5 | ND | ND | ND | 32.6 | 34.5 | |
| B5 | None | ND | ND | ND | 23.0 | ND | ND | ND | 30.9 | 33.1 | |
| B6 | None | ND | ND | ND | 24.1 | ND | ND | ND | 36.0 | 37.9 | |
| B7 | None | ND | ND | ND | 25.7 | ND | ND | ND | 36.1 | 38.1 | |
| B8 | None | ND | ND | ND | 23.0 | ND | ND | ND | 31.5 | 34.0 | |
| B9 | None | ND | ND | ND | 27.0 | ND | ND | ND | 35.8 | 35.9 | |
| Martes foina | F1a | None | ND | ND | ND | ND | 22.5 | ND | ND | 32.5 | 35.3 |
| F2a | Cat/dog | ND | 40.1c | 39.7c | ND | 30.0 | ND | 30.2 | 33.9 | 34.2 | |
| Martes martes | M1a | None | ND | ND | 37.5c | ND | 41.8 | ND | ND | 32.0 | 34.0 |
a
Specimens sequenced by SEQ-PCR for host identification.
b
Other animals present are described for domestic animals living with a different domestic animal(s) in their neighborhood.
c
qPCR was positive once for qPCR performed in duplicate.
d
Samples were removed from the interpretation of the analyses.
e
ND, no amplification was obtained or more than 45 cycles were required. Underlining indicates samples with another carnivore DNA identified in the extract by sequencing.