TABLE 2.
Sequences of PCR inhibitor internal control, primers, and hydrolysis probe for qPCR
| Component | Nucleotide sequence (5′–3′) |
|---|---|
| Alea sequence | GTTCCATGGAAATGCCACCCCGAAGAAACCGCCTAAAAATGTCTATGATTGGTCCACTAAAGTTGATTAAATCAACTCCTAAATCCGCGCGATAGGGCATTAGAGGTTTAATTTTGTATGGCAAGGTACTCCCGATCTTAATGAATGGCCGGAAGTGGTGGATCCTT |
| Alea forward primer | CCTAAAAATGTCTATGATTGGTCCACTA |
| Alea reverse primer | GGGAGTACCTTGCCATACAAAATT |
| Alea probe | VIC-TTAAATCAACTCCTAAATCCGCGCGATAGG-TAMRAa |
a
TAMRA, 6-carboxytetramethylrhodamine.